DNA extraction from plant is crucial step in molecular genetics and for this CTAB protocol is developed which is cationic detergent and CTAB can’t be denatured at higher temperature 60ºC to 65 ºC that is one of the advantage of using CTAB in plant DNA extraction. Due to presence of complex polysaccharides like polyglycosides and other secondary metabolites like tannins, alkaloids and polypheols in plant tissues it is difficult to quantify and qualify pure plant DNA though they are also precipitated with isolated plant DNA. Therefore day by day certain modifications are carried out in CTAB protocol depending upon polysaccharide and other polyphenol composition of individual plant tissue material. Present study explains standardization procedure for quantification and qualification of DNA from leaves of Phyllanthus emblica, Tamarindus indica, Cambopogon citrates, Borhevia diffusa and Bryophyllum pinnatum. Leaf tissues of these plants contain high amount of polyphenols, secondary metabolites like tannins, flavanoids and alkaloids and complex polysaccharides like polyglycosides and more or less amount of Ascorbic acid and citric acid. DNA extraction from Ascorbic acid or Vitamin C rich tissues is bit difficult due to its lower pH that leads to degradation of DNA. So to deal this issue we standardize one small protocol that don’t need use of Rnase enzyme and liquid nitrogen but require frequent pH monitoring during incubation step. DNA isolated from this plant by this protocol is of good quality and quantity and are effective enough to amplify in multiple copies.