Hopea ponga (Dennst.) Mabberley is an endemic and endangered tree belonging to family Dipterocarpaceae, is economically important as timber and reported with several active compounds that have effective as antioxidant and antimicrobial activities. Due to its high economic and medicinal value, the species is over exploited and has become rare and endangered and need high conservation protocols. The present study includes physiological (fresh and dry weight, moisture content and germination) and biochemical changes (total soluble sugars, total soluble protein, lipids, total phenols) during seed development in Hopea ponga. Mass flowering of H. ponga occurred during the month of February and extended upto April followed by mass seed set, that took two months for its maturation. The majority of individuals have developed their seeds in the month of April to June and dispersal occurs with the help of wind. Changes in fresh and dry weights, % MC showed a sigmoidal pattern during seed development and maturation. Total soluble sugar and protein recorded an increase with the seed development while starch and phenols were followed the sigmoidal pattern and were significant as per calculated r².