Rauvolfia serpentina(L.) Benth. ex Kurz is commonly known as Sarpagandha is an important medicinal shrub of family Apocynaceae. This plant species is of great medicinal importance to treat cardiovascular diseases, hypertension, diabetes, malaria, cancer etc. The natural habitat of this species is decreasing due to anthropogenic activities and rapidly eroding natural ecosystem. This plant has comparatively moderate propagation rate in nature. Present investigation is an effort to establish Rauvolfia serpentina for direct and indirect organogenesis from nodal and leaf explants. Leaf and shoot explants were cultured on MS media supplemented with different concentrations of NAA, 2, 4-D, BAP and KIN were used either in singly or in combination. Among all the growth hormones 2, 4-D was the best for callus induction (94% in stem and 98% in leaf) and in combination 2, 4-D and BAP (84% in stem and 94% in leaf). Day of callus induction started from13th to 37th day. For direct regeneration from nodal segment, best growth of auxiliary shoot was obtained on MS medium supplemented with 1.5mg/l BAP and 0.5 mg/l NAA concentrations.

Keywords: Rauvolfia Serpentina; Callus; In Vitro Propagation