Microbial drug resistance is increasing worldwide and is currently considered as major threat to human healthcare. To overcome this problem, several new drugs are being tried to manage and to control the pathogenesis of these microorganisms. Currently, natural sources are being investigated for novel antibiotics. Medicinal plants are good sources of bio-active compounds and chemical structures that have potential beneficial effects. Azadiracta indica (Neem) is a multipurpose tree with more health benefits; the plant shows antimicrobial effects. Azadiractin (AZA) is one of the main bio-active compounds present in neem. AZA shows anti malarial activity, and anticancer activity. Ocimum sanctum L (Tulasi) has anti-stress and anti-oxidant activity. Eugenol (EUG) bio-active compound of Tulasi, has therapeutic potentials in cardio vascular system, central nervous system, blood bio chemistry, and immune system. The main objective of this study is to check the antibacterial activity of bio-active compounds extracted from neem and Tulasi against HAMRSA.