Many plants used today were well known to the people of ancient cultures throughout the world and they were valued for their preservation and medicinal powers. Similarly, Flowers are an essential part of our life, and are wonderful gift of nature, act as a refresher and soothing agents. They are used in Naturopathy and Aromatherapy. The present study deals with the extraction of crude drug from floral samples of four different genera i.e., Buddhleja madagascariensis Lam., Dendranthema grandiflora, Gladiolus alaska, Callistemon citrinus(Curtis) Skeel for evaluation of phytochemical and antibacterial activity. Phytochemical screening of these extracts showed the presence of alkaloids, carbohydrates, flavonoids, protein and phenolic compounds while absence of saponin, amino acids in some of samples. Antimicrobial activity studies revealed that Callistemon citrinus(Curtis)Skeel plant extract had maximum activity against all the studied bacterial strains. Therefore, Minimum Inhibition Concentration (MIC) of Callistemon citrinus(Curtis) Skeel was determined which showed maximum MIC value against B. subtilis followed by S. typii. On the basis of present studies, it was concluded that plants not having only ornamental values but they can be used for extraction of phytochemicals like alkaloids, flavonoids and phenolic which can be used as antioxidants and antibacterial agents. Thus, in near future these floral extract may be use for the welfare of mankind.