Micropropagation technique is being utilized for the production of large scale planting materials through tissue culture. Adansonia digitata commonly known as Baobab, is dioecious plant and therefore, general seeds formation depends upon the plantation of both the plant in the same garden. In Turki, at Muzaffarpur district, Bihar a single plant of Adansonia digitata is growing and even the oldest person of the area are not sure about its exact age. The plant is in such a condition that any time it can be uprooted and the germplasm of this tree shall be eroded. In the present research its micro propagation has been carried out. Here MS (Murashige and skoog 1962) basal medium was supplemented with various concentration of BAP either alone or two different concentration of NAA. Kinetin at various concentration either alone or in combination with NAA was used. It was supplemented with 3% sucrose and gelled with 0.8% agar. The nodal explants were prepared for inoculation through general method of preparation. It was inoculated in the culture medium and was incubated in the cultural room maintained at 26 ^oC and 300 lux light through fluorescent tube. After 20 days it was observed that highest response with respect to initiation of axillary bud was at 3.0 mg/l + antioxidants + 0.2 mg/l NAA. The percent response at this condition was 92 while the mean number of shoots was 2.60 and mean length of shoots was 2.12 cm after 40^th day of inoculation. Well grown tissue cultured raised plantlets were used for rooting in vitro. Half strength MS basal medium supplemented with 4.0 mg/l NAA promoted maximum rooting where the mean number of root per plantlets was 2.0 among 56 % of the explants after 28 days of incubation.