Research Article
Determination of Total Flavonoid, Antioxidant, Antidiabetic, and Antibacterial Activities of Flavonoids Present In Memecylon malabaricum (C.B.Clarke) Cogn
Prathap HM*, Vadlapudi K, Manjunatha T, Ruksana F, Vishala E and Savitharani M
Department of Studies in Biochemistry, Davangere University, Shivagangothri, Karnataka, India
*Corresponding author:Prathap HM, Department of Studies in Biochemistry, Davangere University, Shivagangothri, Karnataka, India. E-mail Id: veeke2012@gmail.com
Copyright:©Prathap HM, et al. 2024. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Article Information:Submission: 21/01/2024; Accepted: 12/02/2024; Published: 14/04/2024
Abstract
Flavonoids are the secondary metabolites that are found in the plants which possess the various biological properties. The present study deals with the total flavonoid content, antidiabetic, antioxidant and antibacterial activities of Leaf flavonoid fraction (LFF) isolated from M. malabaricum. Flavonoid were
isolated and quantified as previously described. Antidiabetic, antioxidant and antibacterial activities were evaluated for Leaf flavonoid fraction (LFF) of M.malabaricum leaves. The Total Flavonoid content was found to be 553.5865±18.56 mg CE/g. IC50 of LFF for α-amylase enzyme and α-glucosidase enzyme
183.95 and 37.720 μg/ml respectively. At 100 μg/ml concentration, the scavenging activity of LFF reached around 54.46% for DPPH and 58.32% for Nitric oxide. While the LFF has significantly inhibited both gram-negative Pseudomonas aeruginosa and gram-positive Staphylococcus aureus bacteria by showing
the maximum zine of inhibition of 8.33 ± 1.15 and 9.66 ± 0.57 respectively. These results exhibited that the flavonoids in LFF have the potential Antidiabetic, antioxidant and antibacterial properties.
Keywords:M. malabaricum; Flavonoid Content; Antidiabetic Activity; Antioxidant Activity; Antibacterial Activity