Vitamin D deficiency (VDD) prevalence has been addressed in many studies covering all continents and having enormous public health consequences. Though VDD is highly prevalent in India, there is paucity of data about it among population in Western regions which receive ample sunlight round the year. Hence this cross-sectional research attempted to investigate the vitamin D levels and the probable determinants in apparently healthy Indians from urban Vadodara in Gujarat state. About 129 subjects (30-60 years of age) from five zones of Vadodara were enrolled through snow-ball effect. Along with informed consent, demographic data and risk factors of VDD were gathered using a semi-structured questionnaire. Fasting blood samples of the participants were collected, and the serum was used to analyze vitamin D by ELISA. Serum 25(OH) D levels of more than equal to 75 nmol/L i.e. ≥30 ng/mL were considered sufficient while levels less than 75 nmol/L were reported as insufficiency or deficiency. The data was subjected to appropriate statistical analysis using SPSS v20. The results revealed that almost 88% of the subjects had VDD, with significant high prevalence among females (p<0.01). Percent body-fat, LDLcholesterol and thyroid hormones- TSH & T3 showed significant negative correlation with the vitamin D levels, while hemoglobin was positively correlated. These along with age emerged as significant predictors for the vitamin D status in the multivariate regression model. Thus it was concluded that VDD was high among the population. So, there is a need to address the predictors for low vitamin D status by adopting a healthy dietary pattern and an active lifestyle with adequate exposure to sunlight.